Understanding and Using Isothermal Gene Assembly for Synthetic Biology
News Aug 28, 2012
Integrated DNA Technologies (IDT) provides a unique insight into the fundamentals of isothermal synthetic gene assembly in this quarter’s edition of the company’s Decoded newsletter.
The technique, which was pioneered in 2009 by Daniel Gibson and colleagues at The J. Craig Venter Institute in Maryland, USA, allows several overlapping DNA fragments to be combined in a single reaction.
In this way, large synthetic DNA molecules many hundreds of kilobases long can be generated using a minimal number of enzymatic steps.
This limits potential errors due to repeated handling of samples and reagents, while simplifying the process by avoiding the need for multiple experimental setups.
The article in the latest issue of Decoded outlines the theory behind isothermal assembly, and provides advice for designing and creating synthetic gene constructs using the method.
Users can read more about the latest genomic trends, tips and research in the most recent issue of IDT’s quarterly newsletter, Decoded.
IDT has also released its new gBlock Gene Fragments, which simplify, speed up and reduce the cost of creating large, custom DNA constructs.
Herpesvirus and Alzheimer's Link: High abundance of Herpes genes in postmortem Alzheimer's brain tissueNews
Data from three different brain banks to suggest that human herpesviruses are more abundant in the brains of Alzheimer's patients and may play a role in regulatory genetic networks that are believed to lead to the disease.READ MORE
Gene-edited Pigs are Resistant to Billion-dollar VirusNews
Scientists have produced pigs that can resist one of the world’s most costly animal diseases, by changing their genetic code. Tests with the virus – called Porcine Reproductive and Respiratory Syndrome, or PRRS – found the pigs do not become infected at all. The animals show no signs that the change in their DNA has had any other impact on their health or wellbeing.READ MORE
Gene Editing Technology May Improve Accuracy of Predicting Heart Disease RiskNews
Scientists may now be able to predict whether carrying a specific genetic variant increases a person’s risk for disease using gene editing and stem cell technologies.READ MORE