We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Using CRISPR To Increase the Shelf-Life of Melons

Gene edited melons cut in half.
Melon fruits 14 d after harvest (stored at 25°C). Credit: University of Tsukuba.
Listen with
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: 1 minute

The gaseous plant hormone ethylene has been long known to promote fruit ripening and play a certain role in shelf-life. In this study, the researchers performed gene editing using the (Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system via modification of the ethylene synthesis pathway in the Japanese luxury melon (Cucumis melo var. reticulatus "Harukei-3") to increase its shelf-life.

Want more breaking news?

Subscribe to Technology Networks’ daily newsletter, delivering breaking science news straight to your inbox every day.

Subscribe for FREE
The enzyme 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) is associated with the final step of the ethylene production pathway and has multiple homologous genes. The research group has previously demonstrated five CmACO genes (homologous genes of ACO) in the melon genome and shown that the CmACO1 gene is predominantly expressed in the harvested fruit. Therefore, we expected that CmACO1 would be an important gene for enhancing the preservation of the melon fruit. In this study, we selected CmACO1 as a target of gene editing and attempted to introduce mutations in the gene. Consequently, the harvested melons exhibited no foreign genes and the mutations induced were inherited for at least two generations. In the non-gene-edited line (wild type), ethylene generation was observed in the fruit 14 days postharvest, the rind turned yellow, and the flesh softened. However, in the genome-edited mutant, ethylene generation was reduced to one-tenth of that in the wild type, with the skin color remaining green and the fruit remaining firm. This indicates that introducing CmACO1 mutation via gene editing enhanced the shelf life of the melons. The results of this study indicate that gene editing can contribute to food loss reduction and improve food security.

Reference: Nonaka S, Ito M, Ezura H. Targeted modification of CmACO1 by CRISPR/Cas9 extends the shelf-life of Cucumis melo var. reticulatus melon. Frontiers in Genome Editing. 2023;5. doi: 10.3389/fgeed.2023.1176125

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.