Webinar: the Cutting-edge qPCR Approach to Single-cell Expression Profiling
News Jun 24, 2013
Integrated DNA Technologies (IDT) has teamed with Professor Mikael Kubista, a pioneer of advanced qPCR methods, to host an informative webinar complete with industry-leading techniques and advice.
The webinar entitled ‘Single Cell Expression Profiling - Breaking Down Biological Complexity’ will be held on 25 June at two different times (0700 and 1300 Central Standard Time), and will detail the novel qPCR-based workflow developed by Professor Kubista for the expression profiling of single cells.
Attendees will find out about the important experimental design aspects and gain first-hand insight into the work of Professor Kubista’s group, including their research into the activation of astrocytes following brain trauma in mice.
The use of single-cell expression profiling has revealed a surprisingly large variation in the number of transcripts among cells from seemingly homogenous tissue, and also between same cell types in culture, emphasizing the risk of measuring combined expression profiles and averaging the responses to environmental changes.
Instead, the webinar explains how single-cell expression profiling provides researchers with a method to explore the true biological properties of cells in a sample, enabling distinct gene expression profiles for distinguishing between cell types, subtypes and subpopulations of cells.
The webinar will also discuss intracellular expression profiling and multianalyte single-cell profiling, allowing the simultaneous measurements of DNA, mRNA, microRNA, IncRNA and protein in the same cell.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.