Webinar: the Cutting-edge qPCR Approach to Single-cell Expression Profiling
News Jun 24, 2013
Integrated DNA Technologies (IDT) has teamed with Professor Mikael Kubista, a pioneer of advanced qPCR methods, to host an informative webinar complete with industry-leading techniques and advice.
The webinar entitled ‘Single Cell Expression Profiling - Breaking Down Biological Complexity’ will be held on 25 June at two different times (0700 and 1300 Central Standard Time), and will detail the novel qPCR-based workflow developed by Professor Kubista for the expression profiling of single cells.
Attendees will find out about the important experimental design aspects and gain first-hand insight into the work of Professor Kubista’s group, including their research into the activation of astrocytes following brain trauma in mice.
The use of single-cell expression profiling has revealed a surprisingly large variation in the number of transcripts among cells from seemingly homogenous tissue, and also between same cell types in culture, emphasizing the risk of measuring combined expression profiles and averaging the responses to environmental changes.
Instead, the webinar explains how single-cell expression profiling provides researchers with a method to explore the true biological properties of cells in a sample, enabling distinct gene expression profiles for distinguishing between cell types, subtypes and subpopulations of cells.
The webinar will also discuss intracellular expression profiling and multianalyte single-cell profiling, allowing the simultaneous measurements of DNA, mRNA, microRNA, IncRNA and protein in the same cell.
Drug Transport Gene May Explain Why Ovarian Cancer Patients React Differently to ChemotherapyNews
A gene which produces a protein that transports drugs in and out of cells may explain why some women treated with chemo have serious side effects.READ MORE
DETECTR Sniffs Out DNA Signals Using CRISPR-Cas9's CousinNews
A new technology allows amplification and detection of genomic snippets present in a sample, using the enzyme Cas12.READ MORE
SHERLOCK, the Paper-Based DNA Sleuth, Receives a Powerful UpgradeNews
SHERLOCK, a CRISPR-based test which allows detection of any genomic signals of interest, is now more portable and powerful.READ MORE