Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)
Poster Mar 05, 2012
Niaz Mahmood, Salma Sultana Alam, Rifit Ara Najnin, Nurun Nahar Fancy, Haseena Khan.
Plant disease resistance genes, mainly known as the R genes, play a vital role in plant defence system through encoding proteins for pathogen detection. Most plant R genes have a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) region. The NBS region is preceded either by a coiled-coil (CC) domain or a TIR (Toll/interleukin-1 receptor like) domain. With a view to identifying and characterizing all LBS domain containing genes in jute, degenerate primers were designed from the conserved MBS domain. Subsequent cloning and sequencing identified the presence of both CC-NBS-LRR and TIR-NBS-LRR domain containing disease resistance genes.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE