Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)
Poster Mar 05, 2012
Niaz Mahmood, Salma Sultana Alam, Rifit Ara Najnin, Nurun Nahar Fancy, Haseena Khan.
Plant disease resistance genes, mainly known as the R genes, play a vital role in plant defence system through encoding proteins for pathogen detection. Most plant R genes have a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) region. The NBS region is preceded either by a coiled-coil (CC) domain or a TIR (Toll/interleukin-1 receptor like) domain. With a view to identifying and characterizing all LBS domain containing genes in jute, degenerate primers were designed from the conserved MBS domain. Subsequent cloning and sequencing identified the presence of both CC-NBS-LRR and TIR-NBS-LRR domain containing disease resistance genes.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE