Latest Posters
Poster
Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)
Study aims include identification and characterisation of all NBS containing genes in jute using degenerate primers designed from the conserved NBS domain, by using two species of jute; susceptible and wild type resistant varieties.
Poster
Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.
Poster
Automation of a Generic Fluorescence Methyltransferase Activity Assay
Epigenetic processes are attracting considerable attention in drug discovery as their fundamental roles in controlling normal cell development and contributions to disease states become more clearly defined. This work combines a fluorescence-based assay with liquid handling and dispensing instrumentation and a multi-mode reader which can be used to monitor the biological activity of the histone methyltransferase (HMT) G9a, a model system.
Poster
Screening for Optimal Algal Cell Growth and Neutral Lipid Production Conditions in Microplates
Algae based products offer many potential advantages over food based sources of energy, however significant research is still required in order to make them an economically viable option. To date, algal research has not extensively utilized the tool of high throughput screening using microplates that has been successfully employed in fields such as drug discovery.
Poster
Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
Poster
In Silico Identification of Conserved Domains from EST Database in Safflower
Identified conserved domains or gene families from the ESTs could be utilised for the identification of gene-targeted markers and for development of functional markers to accelerate marker assisted backcross breeding in safflower.
Poster
Global Gene Expression Changes Induced In Primary Human Hepatocytes By Thiazolidinediones Upon Repeat Dosing of HepatoPac™ Cultures
An assessment of global gene expression changes in HepatoPac, a micropatterned co-culture of hepatocytes and stromal cells.
Poster
Novel colorimetric detection method for the cost-effective identification of influenza on a low-density microarray
The presented work explores the use of ampliPHOX, a rapid, cost-effective, field-amenable colorimetric detection method, combined with the FluChip low-density microarray for screening and surveillance of influenza viruses, particularly in resource-limited settings. Analysis of microarray data from over 100 influenza samples confirmed the ability to easily distinguish influenza A and B and produce unique array patterns for a wide variety of influenza A subtypes.
Poster
Is it possible to quantify and rank the quality of several lists of significant genes found with gene expression profiling by different methods?
High Performance Chip Data Analysis (HPCDA) improves expression profiling of Affymetrix chips via Bioretis database: you can easily use the default parameters and are sure to get the optimum of true positive results, independant of number of significant genes in your dataset. Gene List Significance Index (GLSI) quantifies quality of gene lists. With GLSI its easily judged which normalization/analyzing method gives better results. HPCDA score ranks by relevance.
Poster
Identification of novel autoantigensin patients with liver autoimmune diseases by Protein MicroArray
The characterization of autoimmune disease-specific biomarkers are of primary importance for the development of diagnostic tools and the comprehension of pathogenetic mechanisms leading to autoimmunity. To this aim a protein microarray was employed to analyze serum samples from patients with autoimmune hepatitis (e.g. AIH & PBC) and of healthy as controls. A panel of autoantigens able to discriminate among the groups of patients was identified for potential use as biomarkers.
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