Latest Posters
Poster
Intronic polymorphisms in Daucus carota AOX2b generate putative genotype specific miRNA
A study in the carrot alternative oxidase gene DcAOX2b from several individual plant genotypes of D. carota cv. Rotin revealed the frequent occurrence of intron length polymorphisms (ILPs). Here we will present an in silico analysis performed in order to identify putative miRNA sites at three different sizes of intron 1. The overall research approach aims to develop functional marker candidates for carrot plant breeding.
Poster
SuNS microarray as novel biosensor for clinical diagnostics.
We here present an innovative microarray platform which aims to produce an higher standard of clinical microarray technology. The fabrication approach, involving a novel printing technology (Supramolecular Nano-Stamping) to reduce the production costs of the chips, together with a peculiar probe design, aiming to improve the assay sensitivity and workflow, intend to make the SuNS microarrays well poised to become an important element in the clinical diagnostics market.
Poster
Designing a microarray sensor platform for multiplexed transcriptional library
Intracellular signalling is achieved through complex networks of pathways that ultimately control transcription factor (TF) activity. Deconvoluting such complexity requires a systems level approach. To enable this we have constructed a library of plasmids carrying a TF binding site upstream of a unique reporter sequence. We have designed and optimized an array based readout, which is sensitive, robust and reproducible, to quantify TF derived dynamic changes within cells.
Poster
Altering microRNA miR-15a/16 Levels as Potential Therapy in CLL: Extrapolating from the de novo NZB Mouse Model
Expression of miR15a/16-1 was increased using lentiviral delivery (in vitro and in vivo) or by BSAP knockdown to inhibit B-CLL malignancy.
Poster
Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements
Peripheral blood is widely used as starting material for biomarker discovery and validation using molecular biology technologies. The vast majority of currently published transcriptome data is based on RNA derived either from stabilized whole blood or peripheral blood mononuclear cells (PBMCs). Here, gene expression profiling studies and SOPs for fast, easy and specific manual or automated isolation of monocytes directly from whole blood are being described.
Poster
Demethylation and Re-Expression of Tumor Suppressor Genes: A Novel Approach for Cancer Therapy
In this study, we demonstrated that a combination therapy using suboptimal doses of HDACi and calpeptin, an inhibitor of calpain, produced synergistic type growth inhibition and reduced cancer cell motility in cancer cells. We hypothesize that the re-expression of tumor suppressor genes by demethylation and other mechanisms sensitize the cells and allows for apoptotic death.
Poster
Integrated In Silico Analysis of NGS Prostate Cancer Data via High-Resolution RNA-Seq Analysis
The goal: To get novel insights into the mechanisms of prostate adenocarcinoma by leveraging next generation sequencing (NGS) data, particularly human transcriptome data, through in silico data analysis and interpretation by Ingenuity’s IPA® software. CLC Genomics Workbench and CLC Genomics Server helped us assess short read RNA-Seq data.
Poster
Development and NDA-Level Validation of a Real-Time PCR Procedure for Detection and Quantification of Residual E.coli DNA Contamination of Biopharmaceutical Products
Escherichia coli (E. coli) has been commonly used for the production of biopharmaceuticals. Among the impurities that must be monitored in biopharmaceuticals is residual host-cell DNA (HCD). This study describes the development and subsequent NDA-level validation of a real time PCR procedure developed in response to a client’s need to improve the sensitivity of detection and quantification of residual E. coli HCD in their drug product.
Poster
Neurocognitive correlates of miRNA expression in the CNS of HIV positive subjects with a history of methamphetamine abuse
Methamphetamine (meth) abuse and HIV infection are public health risks that in combination produce a “double epidemic.” MicroRNAs (miRs) were shown to be involved in CNS development, neuronal homeostasis, and brain disease. Past studies linked miR124 and let7-d with cocaine addiction. Our goal was to determine whether miRs were differentially expressed in HIV-infected individuals with a recent history of meth abuse and to identify the neurocognitive correlates.
Poster
Targeted gene silencing of the MAPK pathway in acute myeloid leukemia cells using RNAi
This study explores the potential of multiple gene knockdown in acute myeloid leukemia of pivotal genes controlling the MAPK pathway using RNA interference. Results demonstrate that blocking a major signaling pathway is more complicated than just knocking down the expression of a few genes.
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