Latest Posters
Poster
Digital Volumetric Imaging of Angiogenesis in Tissue Engineered Constructs
Accurate identification of functional vasculature is of critical importance in evaluating construct integration. Digital volumetric imaging (DVI) is an automated serial sectioning and microscopic imaging technique that can bridge the resolution gap between micro-computed tomography and conventional histology. We use DVI to study in vivo vascularization of tissue engineering constructs in a variety of settings.
Poster
Optical Imaging of Cerebellar Dysfunction in SCA1 Mice
Spinocerebellar ataxia type 1(SCA1) is an inherited cerebellar neurodegenerative disorder caused by a polyglutamine tract expansion in the ataxin-1 protein.
Poster
Training rural Guatemalan midwives to assess newborn gestational age
Half of rural Guatemala's population is indigenous; most births are attended at home by traditional midwives. Perinatal mortality is 2nd highest in Latin America. We administered a survey and implemented a training aimed at reducing perinatal mortality.
Poster
Study of an inducible lysis device in E. coli
In synthetic biological systems, cell lysis can be used to engineer programmed cell death and also to release proteins in extra-cellular environment, when secretion is not feasible. In this work, a promoterless lysis device called BBa_K112808, present in the Registry of Standard Biological Parts, was studied. A 3OC6-HSL inducible promoter was cloned upstream of the lysis device and used to characterize it.
Poster
Validation of a standard approach for quantitative characterization of promoter strength
The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.
Poster
Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.
Poster
Hot Start dNTPs - A Novel Tool for Controlled Nucleotide Incorporation in PCR
PCR is a widely used scientific tool employed by a variety of applications. Various Hot Start technologies have already been developed using modified PCR components to increase specificity of a reaction. Recently developed CleanAmpTM dNTPs are modified nucleoside triphosphates with a thermolabile 3’-tetrahydrofuranyl protecting group that is released at higher temperatures. These modified dNTPs prevent low temperature primer extension, which can often be a significant problem in PCR. At higher t
Poster
Chemically Modified Primers for Improved Multiplexed PCR
Multiplex PCR is an advantageous technique used in PCR applications to amplify multiple targets in a single reaction. As useful as it is, this technique presents a new set of challenges that further complicates PCR setup. For example, reactions are more prone to off-target amplifications such as mis-priming and primer dimer due to the increased number of primer pairs. Furthermore, preferential amplification of certain targets leads to an unequal distribution of amplicon products, making quantifi
Poster
Biomass and Quercetin Accumulation Predict Drought Sensitivity in a Novel White Clover Hybrid
Previous research showed that levels of the flavonol quercetin (Q) were positively associated with UV stress resistance in populations of white clover (Trifolium repens L.), and inversely linked to dry matter (DM) production (Hofmann et al, 2000). Q, and to a lesser degree its precursor kaempferol (K), have been implicated as antioxidants and energy dissipating compounds in plants exposed to stress, and could be useful markers for resistance to other stress factors.
Poster
Biomass and Quercetin Accumulation Predict Drought Sensitivity in a Novel White Clover Hybrid
This study shows for the first time that drought resistance in a new white clover hybrid can be linked to drought-induced increases in quercetin levels, and quercetin-to-kaempferol ratio levels. Subsequent DNA-marker analysis studies will be used to identify quantitative trait loci (QTL) for improved drought resistance in white clover to combine high Q response with high herbage yield.
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