Latest Posters

The aim of present study was focused on the effect of silencing target gene in triggering of apoptosis in breast cancer MCF-7 cells. Flow cytometry, light-, confocal microscopy and viability/cytotoxicity tests were used for evaluation of the protein level, percentage of apoptotic cells and morphological features of cell death.

This project focuses on bypassing difficulties and disadvantages of classical homologous recombination to generate animal models with custom genetic alterations, by developing alternative novel and promising approaches for semi-high throughput gene targeting or gene silencing in vivo. We have optimized procedures for construct design, transfection, gene delivery and genotyping. We are evaluating these novel strategies to study genes involved in angiogenesis.

TRANSAT is a research partner for conception of cellular models. Its expertise lies in manipulation of gene expression (RNAi) and in mammalian cell culture. This expertise enables the company to propose functional genomics tools and customized research services, adapted to its clients' problematic. TRANSAT maintains its innovation through R&D programs.

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.

MicroRNAs (miRNAs) comprise a recently identified class of small, non-protein-coding regulatory molecules that play important roles in many physiologic and pathologic processes, including differentiation, viral infection, and oncogenesis. In cancer, abnormal miRNA expression suggests that these molecules may serve as valuable diagnostic and prognostic molecular signatures.

Development of siRNA carriers for in vivo use is an issue, since known in vitro carriers lose their efficacy in vivo, due to poor complex-stability in serum. This complex-stability is difficult to quantify at nanomolar levels, realistic in vivo. Therefore, we developed a fast and sensitive method for this purpose.

Monodisperse magnetic nanoparticles were produced and subsequently coated with a gold layer by the reduction at surface of a gold precursor in order to develop an integrated detection and “gene fishing” system. Functionalization of the nanoparticles could allow the application of this system to a variety of biomolecules.

Addressing the limitations of previous FRET-based MMP assays, this poster documents the development of immunocapture-based FRET assays that are highly sensitive and specifically detect the activity of a particular MMP in a mixed biological sample. The assay sensitivity can reach picogram amounts of enzyme with a large and linear assay range. The assay can also be used to study the interaction between different MMPs.

A new, 30 micron, mono-sized, polymeric resin has been recently developed that provides high resolution and high capacity for synthetic oligonucleotides. Physical properties of this new resin will be described, including particle size uniformity, ion exchange capacity, pressure stability, and chemical stability. The purification of two synthetic 12mer DNA oligonucleotides will be demonstrated, and will compare yields and purities to those from commercially available anion exchange resins.

In this work multivariate data analysis was used as a tool for determining the taxonomy of thirteen solventogenic strains of Clostridia isolated from Colombian soils. This strategy was selected due to its ability for establishing relationships between individuals (strains) based on its characteristics (variables).