Latest Posters
Poster
deltaDOT Technology Label Free Intrinsic Imaging (LFII™)
UV absorption as a technique to detect and quantify biomolecules is routine in molecular biology. LFII™ is a quantum leap up from this state to one that allows the effective imaging of separating biomolecules in an electrophoretic system. The current Peregrine system is based on standard capillary electrophoresis (CE) of biomolecules such as DNA and proteins, small molecules and larger entities such as bacteria and viruses.
Poster
Gene Experssion-based Prediction and Mechanistic Assessment of Non-Genotoxic Chemical-Induced Hepatocarcinogenicity
To facilitate both prediction and mechanism-based assessment of human cancer risk, a liver gene expression signature was derived from short term experiments in the rat to predict non-genotoxic hepatocarcinogenicity. The signature was shown to classify 47 independent chemicals with 85% accuracy, much greater than other putative early biomarkers.
Poster
High-Throughput RNAi by Reverse Transfection with low siRNA Concentrations
The use of short interfering RNA (siRNA) for knockdown of gene expression has become a powerful tool in molecular and cell biology. Some applications require the use of low siRNA concentrations (less than 5 nM), for example, to decrease the possibility of nonspecific effects. We present data from experiments carried out using a new transfection reagent, HiPerFect Transfection Reagent, which allows efficient gene knockdown with less than 5 nM siRNA.
Poster
Effective Fishing of Characteristic Proteome Fractions and Identification of Biomarkers Therein: Application of VisualCockpit to Multidimensional Chromatogram and MS Data
Characteristic 2D fractions are rapidly identified among thousands using interactive visualization, filtering and data mining with VisualCockpit. Biomarker candidates are found therein after identifying proteins from sequence tags. VisualCockpit correlates their concentrations, reflected by normalized MS peptide peak height sum, enzyme activity and immunoreactivity, to donor conditions.
Poster
Simplifying the Flow of Drug Discovery Data
Regardless of research disciplines, scientists need to easily reach the information pertinent to their research. Ideally this data access is easy. Researchers also need the ability to ‘move the data around’ to gain a better view or different perspective. This data manipulation needs to be straightforward. Incorporating the varying views and information required by different scientific disciplines is a considerable challenge.
Poster
Combined Immune Parameters and X-ray data in Early Prediction of Anti-Tuberculosis Chemotherapy Response
20 tuberculosis (12 slow-responders and 8 fast responders) patients were treated with directly observed short course anti-tuberculosis chemotherapy. Chest X-ray was performed. sICAM-1 and suPAR were measured in serum by ELISA, TNFRs using the luminex technology. General discrimination analysis on selected analytes gave, 91.66% and 87,50% correctly classify fast responders and slow responder respectively. The support vector machine analysis gave 100% correct classification.
Poster
DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures
In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.
Poster
Determination of Interferon-Pathway–Related Gene Induction during RNAi
Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.
Poster
Genomewide Profiling After Gene Knockdown by RNAi
In this study, siRNA-mediated RNAi has been combined with genomewide expression profiling using high-density oligonucleotide probe arrays. This combination of technologies provides a vast amount of information and allows optimization of RNAi experiments by monitoring the effects of siRNA transfection on every gene of the genome. CDC2 was efficiently knocked down using siRNA designed by QIAGEN. After knockdown, expression profiling was performed using Affymetrix® GeneChip® arrays.
Poster
New Methods for Rapid Isothermal Amplification and Detection of Short DNA Sequences
Goal: • Rapid, sensitive, specific, low tech, portable DNA diagnostic device
• Detection of clinical pathogens: SARS pathogen, Streptococcus pneumoniae, HSV I & II and biothreat agents: Bacillus anthracis, Brucella species
• Detection of single nucleotide polymorphisms (SNPs)
• Multiplexed detection format
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