Cloning And Purification of a Putative Repressor Protein from Arthrobacter nicotinovorans Pao1
Poster Jul 24, 2013
Marius Mihasan, Marius Stefan, Hritcu Lucian, Vlad Artenie
Besides the well-characterized pathway for nicotine degradation (Brandsch, 2006), pAO1 carries a gene cluster of a hypothetical pathway for carbohydrate utilization. This cluster consists of ORFs of a transcriptional regulator, of a sugar ABC-transporter and of several putative dehydrogenases and oxidoreductases. Previously, we established that the pAO1 orf39 gene encodes an aldehyde-dehydrogenase (Mihasan, 2010) and orf40 encodes an sugar dehydrogenase. Here we focus on further characterization the ORF32 protein and elucidation of its possible role in the cell. By cloning the gene in the plasmid vector pH6EX3, we were able to express it as a recombinant His-tagged protein and to easily purify it to homogenity.
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE