Combining low and high volume liquid handling capabilities for ADME screening
Poster Jan 18, 2014
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk
Current pipetting technologies allow flexible pipetting only down to ~1 μl with sufficient precision and accuracy. In addition, volumes > 0.5% of DMSO, used as a solvent for compound collections, can inhibit enzyme activities.
To allow assays to be performed faster and using less reagents a fast, high quality nanolitre dispensing system is required.
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
mosquito HTS’s ability to miniaturize assays means much smaller volumes, resulting in saving on resources such as buffers, assay kits, radio-ligands, and enzymes.
In addition, this integration could reduce the volume of DMSO waste and decrease the amount of valuable compound used by up to 16-fold.
Characterizing GPCR Activation Using Automated Live Cell ImagingPoster
Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software.READ MORE
Pyroptosis Detection via Multiplexing Cell Death and Bioluminescent Caspase-1 Activity AssessmentsPoster
Researchers developed a convenient method to confirm pyroptosis. Inflammasome activation triggers rapid pyroptosis. Preventing pyroptosis may shift form of cell deathREAD MORE
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE
Comments | 0 ADD COMMENT
International Conference and Exhibition on Nanomedicine and Nanotechnology
Nov 23 - Nov 24, 2017
11th Edition of International Conference on Proteomics 2018
Mar 22 - Mar 23, 2018