Defining off-target cleavage in a pair of Zinc Finger Nucleases
Poster Apr 13, 2012
K. Mukherjee, D. Carroll
Studies on Zinc Finger Nucleases (ZFN) have shown that they can be toxic in organisms. This is potentially due to ZFN cleavage at multiple off-target sites. In applications of ZFNs in human gene therapy, this off-target cleavage is intolerable. We are attempting to develop a procedure to identify these off-target sites in Drosophila. We can then analyze every new ZFN pair for potential off-target cleavage and select and redesign it to work more efficiently.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Highly Accurate HCV Genotyping by Targeted Next Generation SequencingPoster
The recent fast advancement of next generation sequencing (NGS) technologies allowing for unprecedented speed and accuracy in analyzing viral genomes are opening new ways to further improve diagnostic genotyping of HCV.
Detecting resistance associated variants in HCV and HIVPoster
Objectives: to develop improved detection of clinically relevant viral mutations using ion torrent based NGS in an automated workflow.READ MORE