Development of an Automated Platform for HT Cloning and Expression
Poster Jun 22, 2012
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca
• More and more frequently, research projects in fields like structural biology, protein-protein interaction and vaccinology require cloning, expression and purification of a high number of proteins.
• In the Cloning and Expression Unit at Novartis V&D in Siena we have long been involved in Reverse Vaccinology projects, which requires the production and screening of hundreds of antigens with the aim to select the best vaccine candidates (Rinaudo et al., 2009). Then we decided to exploit automation in order to speed up and potentiate our high throughput (HT) procedures, even under the consideration of the significant advances made in the field over the latter years and successfully implemented to molecular biology techniques (Lien and Lee, 2010; Hughes et al., 2011).
Magnetic-microcontact printing based ECM nano-patterning allows homogeneous controlling of cell growth and behaviorPoster
Magnetic – microcontact printing of extracellular matrix proteins (ECM) results in cell microarrays (CMA) showing homogeneous cell growth and attachment on defined patterns. This greatly reduces bias in the analysis of cell responses to toxins, drugs or other stimuli.READ MORE
Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQPoster
This method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). Analysis time is 10x faster than the current FDA regulatory method, increasing sample throughput, avoided spectral interferences and dramatically increased sensitivity. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.READ MORE
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The analysis of the elemental composition of whiskies provides a host of important information including sample origin and understanding how different whiskey styles are caused by processing equipment and raw materials. Preliminary data analysis of the whiskies showed that element compositions could possibly be used to differentiate samples based on age, type, and region.READ MORE