Enhanced Variant Detection and Sample Complexity Representation
Poster Oct 01, 2019
Zana Kapustina, Aliona Markina, Renata Bruzaite, Karolis Matjosaitis, Giedre Stoskiene, Gediminas Alzbutas, Cris Kinross, Arunas Leipus, Sigita Cinciute and Arvydas Lubys
Whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) data are increasingly being utilized to analyze functional variants and changes in gene expression. This helps in understanding diseases, including susceptibility and drug response.
Costs have significantly decreased over the past 10 years, but there are still challenges.
Download this poster to find out how to combat these challenges and achieve:
- Visual feedback for higher library prep success.
- Faster workflows.
- Reduced bias in genomic interpretation.
- Clean data revealing transcriptome complexity.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE