Evaluation of Different RNA Extraction Methods
Poster Apr 03, 2007
The new model-organism, Macrostomum lignano is a marine flatworm that has an average weight of 350 µg and is 1.2 mm long. RNA-isolation from small quantities of tissue needs optimalisation. Our goal was to isolate RNA from small quantities of sample material.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE