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Genomics Research

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Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA

Poster

Published: August 19, 2016

Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA content piece image

The bacterial CRISPR-Cas9 system has revolutionized the genome engineering world with its efficiency and ease of use. The most common use of the CRISPR-Cas9 technology has been to engender gene knockouts that are generated as a result of imperfect repair of a targeted double-strand DNA break by the non-homologous end joining (NHEJ) pathway.