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How to Perform High Throughput siRNA Transfection
Poster

How to Perform High Throughput siRNA Transfection

How to Perform High Throughput siRNA Transfection
Poster

How to Perform High Throughput siRNA Transfection

Abstract
We demonstrate the optimization of siRNA transfection process for time and costs in a high throughput application with two independent measurements of the RNAi effect: The visual monitoring of Eg5 expression and the quantitative PCR measurement of GAPDH mRNA after transfection of Hela cells. The results show, that 1 wash cycle is sufficient for the removal of any remnants of the transfection complex from disposable tips, making the tips re-usable.
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