Identification of differentially expressed transcripts associated to apomixis in B r a c h ia r ia using cDNA microarrays
Poster Sep 04, 2009
Apomixis is a trait which allows flowering plants to produce seeds by asexual ways. Molecular mechanisms behind this phenomenon are poorly understood. We used cDNA microrrays coupled to substractive libraries to find genes related to apomixis in Brachiaria. Genes related to meiosis and cell division, and some putative transcription factors, were overexpressed in sexual plants. It may indicate that apomixis could be caused by downregulation of these genes.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE