MicroRNA Profiling of Breast Cancer using miRCURY™ LNA Arrays

Introduction

• MicroRNAs (miRNAs) comprise a recently identified class of small, non-protein-coding regulatory molecules that play important roles in many physiologic and pathologic processes, including differentiation, viral infection, and oncogenesis.

• In cancer, abnormal miRNA expression suggests that these molecules may serve as valuable diagnostic and prognostic molecular signatures.

• To study the global miRNA expression profiles in breast cancer, we use the miRCURY™ LNA Array platform, which is based on locked nucleic acid (LNA)-modified capture probes that have uniquely high affinities for miRNA.

• For quantitative validation of new miRNA biomarkers, we apply a novel miRCURY™ LNA qPCR assay.