MicroRNA-23b negatively regulates urokinase and c-met and inhibits migration of human hepatocellular carcinoma cells.
Poster Sep 14, 2009
By bioinformatics we predicted that miR-23b could recognize two sites in the 3’ UTR of uPA (urokinase-type plasminogen activator) and four sites in the 3’ UTR of c-met (hepatocyte growth factor receptor). miR-23b transfections in SKHep1C3 caused uPA and c-met decreased and migration and proliferation inhibition of SKHep1C3; anti-miR-23b transfection in human fibroblasts upregulated uPA and c-met. uPA and c-met shared a common microRNA that negatively regulates their expression.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
RAMclust/RAMsearch: efficient post-XCMS feature clustering and annotation of MS-based metabolomics datasetsPoster
Chromatographically coupled mass spectrometry is a powerful tool for profiling, semi-quantitatively or quantitatively, a breadth of small molecules with sensitivity and selectivity.READ MORE
Strategies for Improving RNAi Screening Success: Using a Ubiquitin-EGFP Assay to Identify Druggable Genes Required for Proteasome FunctionPoster
This poster describes the approaches used to ensure effective delivery of siRNA molecules and to identify robust siRNA controls. It also provides the strategies used to validate compatibility of the RNAi automation platform with the phenotype being analyzed.READ MORE