New Transcripts Identified for S. cerevisiae, S. pombe and Drosophila Using Novel cDNA Cloning
Poster Jun 25, 2007
Unannotated transcripts, including antisense, noncoding, intergenic, and potential regulatory RNAs, were identified in three different eukaryotic model organisms by cDNA cloning and sequencing. A rapid double-stranded cDNA generation system will be described.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE