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Optimized Target Enrichment for Reliable NGS of Fragmented cfDNA

A sample tube labelled with cfDNA
Credit: iStock

Next-Generation Sequencing (NGS) of cell-free DNA (cfDNA) has emerged as a promising strategy for early detection, diagnosis and monitoring of cancer progression. 

However, it’s extremely challenging to reliably capture and convert all the fragmented DNA, especially when present at low concentrations within biological samples. 

This poster describes an optimized target enrichment protocol designed to enhance the capture and conversion of fragmented cfDNA for reliable NGS.

Download this poster to discover how to: 

  • Improve the reliability and sensitivity of cfDNA analysis for cancer research
  • Achieve low variant detection thresholds and high variant calling confidence
  • Maximize duplex, on-target molecule conversion for accurate cancer detection and monitoring
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