Platinum™ II Taq Hot-Start DNA Polymerase: PCR simplified with universal annealing
Poster Apr 01, 2020
Skaistė Valinskytė, Edita Elijošiūtė, Tomas Radzvilavičius, Miglė Mikutėnaitė, Jonas Belevičius, Aistė Serapinaitė, Rasa Sukackaitė
Platinum II Taq Hot-Start DNA Polymerase is designed for fast co-cycling of the PCR assays by two innovative technologies. First, Taq enzyme is enginereered by in vitro evolution for faster DNA synthesis and inhibitor resistance. Second, Platinum II PCR buffer contains isostabilizing molecules, which increase primer–template duplex stability during the annealing step. This enables optimal and specific binding of primers with different melting temperatures at universal annealing temperature (60ºC). As a result, the need to optimize the annealing temperature for each primer pair is eliminated. Instead, different assays can be run using the same primer annealing temperature and the same elongation time for amplicons of different lengths.
For circulating cell free DNA (ccfDNA) to be used in cancer research successfully, workflow standardization is essential. Access this poster to discover tips on optimal workflow control, how to yield smaller ccfDNA fragments and the differences in quantification and qualification of ccfDNA.READ MORE