Potential Use of RNAi on Human Skin
Poster Dec 01, 2008
We designed highly efficient StealthTM RNAi targeting human tyrosinase with optimum IC50 in the 10-100 pM range. By silencing tyrosinase in melanocytes, we achieved the reconstruction of an artificial epidermis showing a long term lasting (>30 days) inhibition of melanogenesis. Since skin pigmentation can phenotypically be monitored, the use of StealthTM RNAi against tyrosinase on reconstructed pigmented epidermis is a precious tool for the evaluation of delivery vehicles.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE