Prediction of Genes with U12-Dependent Introns in the Human Genome
Poster Jun 15, 2007
We enhanced the gene prediction program GeneID with a framework for detecting U12-dependent introns without appreciable loss in gene prediction sensitivity or specificity. We present statistics on the performance of the U12-enhanced GeneID on a test set of known U12-intron-possessing genes as well as results for scans of both the ENCODE regions and the entire human genome.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE