Quantification of siRNA by a Novel Competitive-qPCR Method
Poster Sep 29, 2007
We have developed a competitive qPCR method in which siRNA competes with a homologous forward primer to bind template DNA, giving siRNA concentration dependent inhibition. The addition of E6-siRNA to cqPCR led to inhibition of amplification in a linear concentration-dependent manner, with as little as 200pg of siRNA capable of being detected. Irrelevant siRNA had no effect on amplification confirming specificity.
Characterizing GPCR Activation Using Automated Live Cell ImagingPoster
Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software.READ MORE
Highly Accurate HCV Genotyping by Targeted Next Generation SequencingPoster
The recent fast advancement of next generation sequencing (NGS) technologies allowing for unprecedented speed and accuracy in analyzing viral genomes are opening new ways to further improve diagnostic genotyping of HCV.
Detecting resistance associated variants in HCV and HIVPoster
Objectives: to develop improved detection of clinically relevant viral mutations using ion torrent based NGS in an automated workflow.READ MORE
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