Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes with fatty acid metabolism but not with glucose uptake in L6 muscle cells

The role of RIP140 in the regulation of fatty acid uptake, oxidation and glucose uptake was investigated in muscle cells using RNAi technique. Cells were incubated ±insulin before measurement of glucose and FA kinetics. We suggest that reducing RIP140 expression in muscle has no impact on the regulation of GU but leads to high rates of FAU and reduced sensitivity to insulin as it pertains to FA metabolism.