Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes with fatty acid metabolism but not with glucose uptake in L6 muscle cells
Poster Apr 29, 2010
The role of RIP140 in the regulation of fatty acid uptake, oxidation and glucose uptake was investigated in muscle cells using RNAi technique. Cells were incubated ±insulin before measurement of glucose and FA kinetics. We suggest that reducing RIP140 expression in muscle has no impact on the regulation of GU but leads to high rates of FAU and reduced sensitivity to insulin as it pertains to FA metabolism.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE