Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
Poster Sep 13, 2012
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele
Currently, expression and function of lncRNAs during human disease and development is largely unexplored. Few platforms are available that allow sensitive and specific high-throughput lncRNA expression profiling.
Study concludes, among other things, that lncRNA expression profiling of 60 cancer cell lines representing 9 different tumour entities reveals cancer-specific lncRNA expression profiles.
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Highly Accurate HCV Genotyping by Targeted Next Generation SequencingPoster
The recent fast advancement of next generation sequencing (NGS) technologies allowing for unprecedented speed and accuracy in analyzing viral genomes are opening new ways to further improve diagnostic genotyping of HCV.
Detecting resistance associated variants in HCV and HIVPoster
Objectives: to develop improved detection of clinically relevant viral mutations using ion torrent based NGS in an automated workflow.READ MORE