Validation of a standard approach for quantitative characterization of promoter strength
Poster Feb 28, 2010
The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.