Validation of a standard approach for quantitative characterization of promoter strength

The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.