454 Life Sciences Announces Solution for De Novo Sequencing and Assembly of Complex Genomes
Product News Apr 24, 2009
454 Life Sciences has announced the launch of the GS FLX Titanium Series Library Paired End Adaptors for improved whole genome de novo sequencing and high-quality assembly using the Genome Sequencer FLX System.
Widely recognized as the preferred sequencing platform for de novo applications as a result of the system’s long 400-500 base shotgun reads, the addition of 3 Kb, 8 Kb and 20 Kb span paired end libraries and improved assembler software now facilitate assembly of increasingly complex genomes.
De novo sequencing, which refers to the sequencing of genomes without the use of a reference, has been largely enabled by the technology of the 454 Sequencing System in recent years. The new GS FLX Titanium series paired end solution continues to raise the bar in de novo sequencing by offering biologists more complete genome coverage, assembly into fewer, larger scaffolds and the ability to explore the full range of genetic variation.
The company says that the streamlined processes allow researchers to simultaneously sequence multiple shotgun and paired end libraries in the same 10-hour instrument run. The extra-long 20 kb span reads cover the highly repetitive regions of complex genomes and, in combination with long shotgun reads, essentially eliminate the need for time-consuming fosmids or Sanger method.
Additionally, algorithmic improvements to the GS De Novo Assembler software have increased the speed and power to process larger, more complex genomes.