96 Barcodes for NGS Multiplexing
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The NEXTflex-96 DNA Barcodes accomplish this by providing 96 indexed adapters, each of which contains a unique 8 nt sequence. The indices are designed to be error resistant and allowing for proper differentiation between samples. Using the NEXTflex-96 DNA Barcodes, the index is added to the sample during the adapter ligation step, allowing PCR amplification to be reduced or eliminated, thus minimizing amplification-based bias and preventing poor reads from single base errors introduced during PCR. These barcodes can be used to multiplex genomic DNA, RNA and ChIP sequencing libraries.