Agilent Introduces High-Sensitivity Protein Kit for its Bioanalyzer that Outperforms Silver-Stained Gels

Agilent Technologies Inc. has introduced the Agilent High Sensitivity Protein 250 Kit for the Agilent 2100 bioanalyzer, which is designed to deliver sensitivity than silver-stained SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The kit can also provide quantitation capability along with the other advantages of a microfluidic lab-on-a-chip based kit.

The High Sensitivity Protein Kit, designed for both pharmaceutical QA/QC and life sciences research experiments, detects proteins down to as little 1 pg/ul, comparable to or better than silver-staining slab gels.

The kit features a dynamic range of four orders of magnitude for quantitation and covers a sizing range from 10 - 250 kDa. For QA/QC applications, users can detect a 0.05-percent impurity while visualizing the main target with reliable quantitation.

“This is the latest example of our program to extend the sensitivity, speed, low sample consumption, ease of use and cost savings of the multi-purpose 2100 bioanalyzer system to additional life science applications,” said Knut Wintergerst, Agilent’s Electrophoresis marketing manager. “We think researchers will immediately recognize the advantages of replacing traditional silver-staining slab gels with a faster, more-reproducible and quantitative method.”

The new kit features a direct labeling reaction that is highly reproducible. Separation, quantitation and purity measurements can be performed in a single step, and typical throughput for QA/QC is 10 samples per hour.