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AnaSpec Develops HTS Assay Kits for Screening HCV NS3/4A
AnaSpec has announced that it has developed the HTS assay kits for screening HCV NS3/4a.
EnzoLyte™ assay kits are designed for the detection and quantification of NS3/4A protease activity at subnanomolar concentrations. They can be used in 96- or 384-well microplate format.
There are three assay kits to choose from depending on what wavelength is used for monitoring the cleavage of the FRET substrate.
Convenient mix-and-read format and one-step procedure make EnzoLyte Assay Kits ideal for high-throughput screening.
EnzoLyte™ 490 HCV Assay Kit:
Peptide substrate paired with Dabcyl (fluorophore) and Edans (quencher). Fluorescence can be continuously monitored at Ex/Em=340/490nm. Kit size: 500 assays.
EnzoLyte™ 520 HCV Assay Kit:
Peptide substrate paired with 5-FAM (fluorophore) and QXL™ 520 (quencher). Fluorescence can be continuously monitored at Ex/Em=490/520 nm. Kit size: 100 assays.
EnzoLyte™ 620 HCV Assay Kit:
Peptide substrate paired with HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher). With near red emission wavelength, this FRET peptide avoids the autofluorescence interference associated with most test compounds.
Fluorescence can be continuously monitored at Ex/Em=590/620nm. Kit size: 100 assays.
