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AnaSpec Introduces Dyes for Labeling Amine- and Thiol-Modified Oligos


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AnaSpec highlights its own proprietary HiLyte Fluor dyes for labeling amine and thiol modified oligonucleotides from its wide collection of amine and thiol reactive 6-isomeric dyes of fluoresceins and rhodamines.

Fluorescent dye-labeled oligos are widely used as primers for sequencing and PCR reactions, and also as probes for fluorescence in situ hybridization (FISH). Double-labeled oligos are used to produce fluorescence resonance energy transfer (FRET) for a number of applications such as real-time PCR assays and automated DNA sequencing.

Synthetic oligos can be modified with amine or thiol groups at their 5’-end, 3’-end or internally. These amine and thiol incorporated oligos can then be labeled with amine and thiol reactive fluorophores, respectively.

N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labeling amine-modified oligos. Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labeling thiol-modified oligos.
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