AnaSpec Introduces Fluorescent Calcium Indicators

AnaSpec showcases a wide selection of fluorescent Ca2+ indicators. They include UV-excitable and visible light-excitable calcium indicators.

Among the UV-excitable calcium indicators, Fura-2 and Indo-1 are the most commonly used. Fura-2 is excitation-ratioable while indo-1 is emission-ratioable. While Fluo-3 and Rhod-2 are the most commonly used among the visible light-excitable calcium indicators.

Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based HTS assays for functional GPCR assays. The long-wavelength Ca2+ indicators, Rhod-2 and X-rhod-1, are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high autofluorescence.