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Automation Boosts Sample Preparation for Next-Generation Sequencing

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Tecan has worked with Applied Biosystems, part of Life Technologies Corporation, to develop an off-the-shelf system for automated bead washing, based on Tecan’s Freedom EVO® 75 workstation equipped with a Te-MagS™ magnetic bead separation module.

Automation of ePCR purification for the Applied Biosystems’ SOLiD™ System next-generation genetic sequencing platform reduces the manual sample preparation.

Preparation of templated beads for the SOLiD System is a multi-step process, requiring several washes of magnetic beads for ePCR product purification. In a collaboration between Tecan and Life Technologies, a new system based on the Freedom EVO 75 workstation has been developed to reduce manual variation and improve consistency.

The Freedom EVO 75 bead washing station is equipped with a two-channel LiHa arm, a Te-MagS magnetic separation module and all the racks and carriers required for parallel preparation of 24 templated bead samples.

In addition, the system’s Freedom EVOware® software features washing protocols specifically developed by Life Technologies’ Genetic Systems Division for optimal sequencing performance of the SOLiD System.