We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
BigDye® XTerminator™ Purification Kit for Producing Higher Quality DNA Sequence Data
Product News

BigDye® XTerminator™ Purification Kit for Producing Higher Quality DNA Sequence Data

BigDye® XTerminator™ Purification Kit for Producing Higher Quality DNA Sequence Data
Product News

BigDye® XTerminator™ Purification Kit for Producing Higher Quality DNA Sequence Data


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "BigDye® XTerminator™ Purification Kit for Producing Higher Quality DNA Sequence Data"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Applied Biosystems has released the BigDye® XTerminator™ Purification Kit for producing higher quality DNA sequence data during Sanger Sequencing, while shortening the amount of time and work required for purification.

Sanger Sequencing is considered to be the gold standard method for DNA analysis and part of the workflow includes a clean-up step to remove by-products of the sequencing reaction from the labelled DNA fragments.

Insufficient removal of these by-products can result in shorter sequence read lengths and ‘dye blobs’ that make it difficult to accurately interpret results or obtain high quality data.

The BigDye XTerminator Purification Kit involves a four-step protocol designed to provide more reliable dye blob removal, increased sample stability and more reproducible, higher quality results.

Laragen Inc, a service laboratory that provides DNA sequencing and analysis services for academic and commercial clients, is one of several organisations that tested the new kit.

“We have tried virtually all of the commercially available methods for DNA purification, and the BigDye XTerminator Purification Kit has provided us with the highest quality results we’ve seen and with less manual labour than other methods,” said Dr Jin Li, vice president of Laragen.

“Using this kit, we get very strong and even signals from beginning to end and longer sequencing reads, without dye blobs or lost samples.”

Advertisement