Bioo Scientific Releases 18S ITS rRNA Library Prep Kit
Product News Jan 10, 2015
The NEXTflex™ 18S ITS Amplicon-Seq Kit simplifies the preparation of multiplexed amplicon libraries spanning the ITS1 and ITS2 hypervariable regions of eukaryotic 18S ribosomal RNA (rRNA) genes to permit the fast and cost-effective characterization of uncultured fungal and micro-eukaryotic organisms.
Up to 384 multiplexing barcodes are available with this kit, to greatly reduce the cost of 18S ITS NGS studies on the Illumina® MiSeq platform. Libraries prepared using this kit are compatible with regular Illumina sequencing primers.
NEXTflex 18S ITS amplicon processing involves two main steps: an initial PCR amplification using customized PCR primers that target the 18S ITS domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The number of cleanup steps is limited to ensure maximum recovery of amplicons for downstream sequencing.
The NEXTflex 18S ITS Amplicon-Seq Kit is the latest addition to Bioo Scientific’s line of library prep kits for Illumina-compatible metagenomics analysis, which includes the NEXTflex™ 16S V1 - V3 and 16S V4 Amplicon-Seq Kits.