Bio-Rad Describes Methods for Protein A Antibody Purification
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Bio-Rad Laboratories, Inc. has announced the availability of a technical note (tech note 5712A) entitled, “The Profinia™ Protein Purification System Simplifies Antibody Purification with Protein A”.
The technical note provides results that demonstrate how the Profinia protein purification system is ideal for the purification of antibodies using Protein A. A significant advantage of the Profinia system is its integrated tandem column configuration, which allows for a purified antibody to be exchanged from an acidic elution buffer to a defined neutral buffer of choice.
The system’s preprogrammed methods are fully automated and require less time than traditional gravity-flow or syringe-based methods.
In addition to purification of IgG from serum, the Profinia system is ideal for the purification of monoclonal antibodies from the supernatants of hybridoma cell cultures using immobilized Protein A. Large sample volumes of hybridoma cell culture supernatants are shown to be accommodated by the Profinia system.
To accommodate antibodies from samples that bind poorly to protein A, the Profinia system is compatible with prepacked chromatography cartridges kits, such as those for Protein G, from other suppliers using a simple adaptor.
The Profinia system also provides optional software designed to monitor real-time chromatography parameters. The software also generates publication-quality chromatograms and reports.
Separations carried out on the Profinia system require a minimum amount of chromatography experience and allow researchers to focus more on the downstream applications of the purified product than with the details of the purification process itself.
