We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Bio-Rad Introduces iScript™ Advanced cDNA Synthesis Kit for  RT-qPCR
Product News

Bio-Rad Introduces iScript™ Advanced cDNA Synthesis Kit for RT-qPCR

Bio-Rad Introduces iScript™ Advanced cDNA Synthesis Kit for  RT-qPCR
Product News

Bio-Rad Introduces iScript™ Advanced cDNA Synthesis Kit for RT-qPCR


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Bio-Rad Introduces iScript™ Advanced cDNA Synthesis Kit for RT-qPCR"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

With a maximum capacity of 7.5 µg of input RNA, the iScript Advanced cDNA synthesis kit for RT-qPCR surpasses the capacity of its nearest rival by two micrograms. The ability to use more RNA in a reaction means researchers can:

• Improve reproducibility (a single RT reaction is diluted for qPCR analysis of all target genes, including housekeeping genes)
• Reduce cost per qPCR data point (several-fold higher RNA input with minimal increase of RT cost per reaction)
• Increase sensitivity for certain target genes (low copy targets can be detected within standard number of qPCR cycles)

The iScript Advanced cDNA synthesis kit for RT-qPCR offers additional advantages over competitor kits in terms of simplicity and speed. The kit has only two essential components: an iScript Advanced reaction mix and an iScript Advanced reverse transcriptase. The protocol takes only 35 minutes. By contrast, competitor cDNA synthesis kits contain up to seven components, and some “two-tube kits” recommend an additional optional step, resulting in a protocol that requires an hour or more to complete.

The iScript Advanced cDNA synthesis kit for RT-qPCR offers excellent sensitivity and efficiency in both real-time PCR (using SYBR Green, EvaGreen, or probes) and conventional RT-PCR.

The iScript Advanced cDNA synthesis kit is part of the iScript family of products formulated for efficient and sensitive reverse transcription over a wide linear dynamic range of input RNA for real-time qPCR. Available products include:

• The original two-tube iScript cDNA synthesis kit for reverse transcription of standard total RNA inputs between 1 µg and 1 pg
• The iScript reverse transcription supermix, a streamlined (1 tube) version for improved reproducibility and high-throughput applications
• The iScript Select kit, which enables a choice of priming strategies, including gene-specific priming, for standard total RNA inputs between 1 µg and 1 pg
Advertisement