Bio-Rad Paper Describes Fast Supermixes for Real-Time PCR

Bio-Rad Laboratories, Inc. has announced the availability of a new bulletin that describes iTaq™ fast supermixes with ROX which can enable reliable quantitative PCR results. The bulletin also provides quantitative graphical proof of the efficacy of the assays.

iTaq fast supermixes with ROX are concentrated and ready-to-use reaction mixtures for real-time qPCR on a variety of instruments including ROX-dependent real-time PCR systems. These mixes are optimized to deliver maximum PCR efficiency, sensitivity, precision, and robust fluorescent signal using fast or conventional cycling protocols.

The antibody-mediated and hot-start technology employed by iTaq DNA polymerase, sequesters polymerase activity prior to the initial PCR denaturation step. Upon heat activation, the antibody denatures irreversibly, releasing fully active iTaq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

The ROX internal reference dye included in the product is used for normalization of fluorescent signal and to correct for well-to-well optical variations in ROX-dependent instrumentation. These supermixes provide the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts.

The bulletin (number 5737) can be requested at www.bio-rad.com.