Bio-Rad Releases Bio-Plex Assay Guide Describing Measurement of Human Acute Phase Markers

Bio-Plex Pro human acute phase assays can deliver accurate and reproducible measurements of the nine commonly studied acute phase markers:  a-2-Macroglobulin, CRP, ferritin, fibrinogen, haptoglobin, procalcitonin, SAA, serum amyloid P (SAP), and tissue plasminogen activator – in serum, plasma, and tissue culture supernatant. A snapshot of panel performance is demonstrated by representative parallelism and linearity of sample dilution in normal human serum and plasma.

The assays are offered for research use as two separate kits to achieve optimal working assay ranges. The Bio-Plex Pro human acute phase 5-plex assay requires a 100-fold sample dilution, and the Bio-Plex Pro human acute phase 4-plex assay requires a 10,000-fold sample dilution as required by the physiological ranges of the analytes. Dividing the targets into panels that require similar dilutions reduces the need to expend assay reagents on multiple dilutions of each sample.

For a current listing of Bio-Plex Pro human acute phase assays, visit the Bio-Rad website at www.bio-rad.com/bio-plex/ .  The bulletin (number 5650), which includes a comprehensive human assay ordering guide is available either from a local Bio-Rad sales office or it may be requested by visiting the Bio-Rad web site.