We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells
Product News

Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells

Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells
Product News

Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Bio-Rad Releases Poster that Describes Transfection of Primary and Difficult-to-Transfect Cells"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Bio-Rad Laboratories, Inc. has announced the availability of a poster (number 07-0883) that was displayed at the Association for Biomolecular Resource Facilities Annual Meeting held in February, 2008.

In the poster, Bio-Rad demonstrates how high efficiency electroporation of several difficult-to-transfect cell lines was achieved using the parameter optimization capabilities of the Gene Pulser MXcell™ electroporation system.

Electroporation conditions, including optimal waveform and pulse conditions, were determined in parallel for each cell type. Results of several difficult-to-transfect cell types, including HUVEC (human umbilical vein endothelial cell), Jurkat, and mouse neuroblastoma cells (Neuro-2A) with plasmid DNA or siRNA, are shown.

Data presented in the poster shows that four-hours post electroporation with a GAPDH siRNA, HUVEC cells expressed less than 97% of GAPDH mRNA than controls transfected with scrambled siRNA. In Jurkat cells, significant silencing was also obtained (>88%) as quickly as four hours post transfection and this silencing persisted for 48 hours. Transfection of Neuro-2A cells with a fluorescently labeled siRNA followed by analysis with flow cytometry resulted in 75% transfection efficiency.

Advertisement