Chromatrap® Enriches Epigenetic Marks from Primary Cells
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A new application note from Porvair Sciences details a protocol for its Chromatrap® ChIP assay kit that has been developed to enrich epigenetic marks from primary human tissue cells.
Chromatrap® Pro-A, the novel solid-based matrix for chromatin immunoprecipitation assays (ChIP) has been successfully used for isolation of high quality chromatin from difficult biopsy material.
Focusing on an application to precipitate epigenetic marks from primary endometrial stromal cells - results show high abundant epigenetic marks from 1 million cells isolated from a tissue biopsy, with selective amplification of positive gene targets relative to negative IgG and negative gene loci.
Not only is Chromatrap proven to produce positive amplification of histone regulatory marks at specific gene loci but also the novel ChIP assay kit is shown to be compatible with human tissue.
