We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

Easy and Cost-Effective Generation of siRNAs from Full-Length Target Genes

Listen with
Speechify
0:00
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: Less than a minute

AMSBIO has introduced a Dicer siRNA Generation Kit that mimics the natural RNA interference process by using recombinant human dicer enzyme, a double-stranded RNA-specific endonuclease, to cleave in vitro transcribed dsRNA templates into a pool of 22 bp siRNAs.

The new kit allows easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes. For maximum convenience, the Dicer siRNA Generation Kit contains everything that is required for preparing double stranded RNA, RNA cleavage, siRNA cleanup and transfection.

Using the Dicer kit takes the guesswork out of siRNA generation as preparing a mixture of siRNAs has a better chance of success than a single siRNA design.

Consequently labs using the kit will benefit as they waste less time and money on failed siRNA designs and are able to screen more regions of genes for silencing.

Each AMSBIO Dicer siRNA Generation Kit contains sufficient material for transcribing, cleaving, and transfecting siRNAs for 50 transfection experiments in 24-well plates with up to 5 different genes.