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Enhanced DNA Amplification with new DNAreleasy
Read time: Less than a minute
DNAreleasy from Nippon Genetics Europe is now available in the UK and Ireland from Anachem. This reagent for the lysis of cells and the release of genomic DNA requires just 10 minute incubation on a thermal cycler before the DNA is ready for PCR processing.
DNAreleasy has been successfully used to lyse a wide range of cells. This includes: human buffy coat, human fibroblasts tissue culture cells, human sperm, mouse & rat tissue culture cells, mouse tail, various plants, Drosophila and others like yeast, Gram +ve bacteria, Gram –ve bacteria, viruses and many more.
Using DNAreleasy is easy. Mix cells with 20 µl of the reagent and overlay with mineral oil if necessary. Place in a Thermal Cycler and treat at 75°C for 5 minutes followed by 96°C for 2 minutes before holding at 20°C. After the lysis, a part or all of the DNAreleasy-lysate can be used directly in PCR. DNAreleasy can make up to 40% of most PCR mixes. It can be stored at -20°C for future use.
DNAreleasy has been successfully used to lyse a wide range of cells. This includes: human buffy coat, human fibroblasts tissue culture cells, human sperm, mouse & rat tissue culture cells, mouse tail, various plants, Drosophila and others like yeast, Gram +ve bacteria, Gram –ve bacteria, viruses and many more.
Using DNAreleasy is easy. Mix cells with 20 µl of the reagent and overlay with mineral oil if necessary. Place in a Thermal Cycler and treat at 75°C for 5 minutes followed by 96°C for 2 minutes before holding at 20°C. After the lysis, a part or all of the DNAreleasy-lysate can be used directly in PCR. DNAreleasy can make up to 40% of most PCR mixes. It can be stored at -20°C for future use.
