To continue reading this article, sign up for FREE to
Membership is FREE
and provides you with
instant access
to email newsletters, digital publications, our full content catalogue & more...
EPICENTRE Introduces BACMAX™ DNA Purification Kit
EPICENTRE has introduced BACMAX™ DNA Purification Kit that can enable isolation of exceptionally pure intact BAC DNA without binding columns, resins or organic extractions.
The BAC DNA is suitable for many applications, including sequencing, fingerprinting, PCR and preparation of shotgun libraries. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 µg of BAC DNA from a 1.5 to 100 ml culture of a single-copy BAC.
Selective precipitation steps and the incorporation of EPICENTRE's RiboShredder™ RNase Blend remove contaminants that degrade DNA and interfere with downstream applications.