Fast one-step RT-PCR on any Real-time Cycler
Product News Oct 04, 2007
Qiagen has introduced QuantiFast™ Probe RT-PCR kits to deliver faster results in real-time one-step RT-PCR with sequence-specific probes, such as TaqMan® probes.
The kits are a new addition to the range of QuantiFast Kits, which provide fast cycling on any real-time cycler. With time savings of up to 60% on existing cyclers, there is no need for costly hardware upgrades, the company claims.
A specially developed fast RT-PCR buffer containing the patent-pending additive Q-Bond greatly reduces denaturation, annealing, and extension times. The buffer also contains HotStarTaq® Plus DNA Polymerase, which requires only 5 minutes at 95ºC for activation. The optimized QuantiFast RT Mix enables cDNA synthesis in just 10 minutes.
According to Qiagen, accurate quantification of even low copy numbers is achieved with the kits. A balanced combination of KCl and NH4Cl in the fast RT-PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity. In addition, HotStarTaq Plus DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.