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Genotyping Solutions for Agricultural Biotechnology Applications

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New England Biolabs (NEB®) has announced the launch of the NEBNext Direct Genotyping Solution, representing the first capture-based approach for high-throughput genotyping for a wide variety of applications in agricultural biotechnology.

The NEBNext Direct Genotyping Solution combines highly multiplexed, capture-based enrichment with maximum efficiency and next-generation sequencing (NGS) to deliver a one-day, automatable genotyping workflow. Applicable for marker ranges spanning 100 to 5,000 markers, pre-capture multiplexing of up to 96 samples, in addition to dual-indexed UMI sequencing, allows for more than 3.8 million genotypes in a single Illumina® sequencing run.

Derived from the NEBNext Direct capture-based target enrichment technology, the genotyping solution uses efficient capture probe design, allowing for sequencer efficiency and reduced reagent use, thus further reducing assay costs.

Other features of the product include:

  • Optimized bait design captures both DNA strands
  • Highly specific enrichment, >90 percent of reads mapping to targets
  • Coverage uniformity to avoid over and under-representation with fewer reads
  • Similar performance across DNA from crude and high-quality based extraction procedures

In addition to applications in agricultural biology, the technology can be applied in other areas where lower costs and higher throughput are necessities. Adding sample barcodes and pooling the samples early in the process significantly reduces the number of protocol steps, renders the approach attractive for uses in human and animal genotyping, including opportunities for biobanking, sample quality control for NGS, and other applications in human health and livestock breeding.