High Throughput PCR Plate Preparation
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The automated workstation is shown to provide a reliable, efficient solution for processing plates in batches or one at a time through the necessary stages of PCR plate preparation. Using the described protocol up to 50 diluted replicate plates with Master Mix and Exon additions can be run without user intervention. The typical throughput for the described set-up was found to be about 5 hours for 50 plates, depending on exact protocol and liquid handling steps. Up to 2 additional BenchCel systems can be integrated to the Bravo liquid handler to further increase capacity.
The on-deck accessories for the Bravo liquid handler are shown to provide the temperature control and sonicating wash to decontaminate tips that are necessary capabilities for working with DNA and other sensitive materials. Velocity 11's powerful, yet highly intuitive VWorks automation control software was used to provide a single, simple user interface to control all critical task parameters.
The combination of Velocity11's Bravo™ liquid handler, BenchCel® plate handling system, PlateLoc® thermal microplate sealer and a bulk reagent dispenser is shown to allow users to run the PCR preparation with high throughput and maximum walk-away time.
Since 1999, Velocity 11 has combined innovative engineering with high standards of quality and customer service, to design and manufacture flexible high-performance automation systems and instruments for applications that include medium to high throughput screening of cell-based and enzyme assays, ELISA, plasmid and sequencing preparation, PCR clean-up, solubility, and more.
